Cloned (Comment) | Organism |
---|---|
gene yjeK, construction of polycistronic vectors encoding His-tagged or non-tagged EF-P, with YjeA, a class II lysyl-tRNA synthetase, and YjeK and overexpression in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knockout mutant generation, phenotype, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Escherichia coli | lysyl modification at lysine 34 in native and recombinant bacterial elongation factor-P proteins, no activity with bacterial elongation factor-P mutant K34A | (3S)-3,6-diaminohexanoate | - |
? | |
L-lysine | Escherichia coli K-16 | lysyl modification at lysine 34 in native and recombinant bacterial elongation factor-P proteins, no activity with bacterial elongation factor-P mutant K34A | (3S)-3,6-diaminohexanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
MG1655, gene yjeK | - |
Escherichia coli K-16 | - |
MG1655, gene yjeK | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | - |
Escherichia coli | (3S)-3,6-diaminohexanoate | - |
? | |
L-lysine | lysyl modification at lysine 34 in native and recombinant bacterial elongation factor-P proteins, no activity with bacterial elongation factor-P mutant K34A | Escherichia coli | (3S)-3,6-diaminohexanoate | - |
? | |
L-lysine | - |
Escherichia coli K-16 | (3S)-3,6-diaminohexanoate | - |
? | |
L-lysine | lysyl modification at lysine 34 in native and recombinant bacterial elongation factor-P proteins, no activity with bacterial elongation factor-P mutant K34A | Escherichia coli K-16 | (3S)-3,6-diaminohexanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
YjeK | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | class II lysyl-tRNA synthetase and lysine-2,3-aminomutase are implicated in the modification of bacterial elongation factor P, EF-P, to convert a specific lysine to a hypothetical beta-lysyl-lysine. Both enzymes, YjeA and YjeK, are required forbeta-lysylation of EF-P. beta-Lysyl-EF-P stimulated N-formyl-methionyl-puromycin synthesis 4fold over the preparations containing unmodified EF-P and/or beta-lysyl-EF-P. The mutant K34A lacking the modification site lysine is inactive. YjeA canbeta-lysylate EF-P in vitro or in cells independently of YjeK. In contrast, YjeK alone or supplementation with D-beta-lysine cannot lysylate EF-P | Escherichia coli |